Lung germinal center like B-cells
RNA-seq (gene expression) · mouse · 14 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 14 samples: 14 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Activation and maturation of antigen-specific B cells in nonectopic lung infiltrates are independent of germinal center reactions in the draining lymph nodeCellular & molecular immunology · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE274693_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1147665 and SRA study SRP525112. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 14 sample titles: 14 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8447695 Exp 1 Ln PB
- GSM8447696 Exp 1 Ln GC
- GSM8447697 Exp 1 Lung PB
- GSM8447698 Exp 1 Lung GC-like
- GSM8447699 Exp 2 Ln PB
- GSM8447700 Exp 2 Ln GC
- GSM8447701 Exp 2 Lung PB
- GSM8447702 Exp 2 Lung GC-like
- GSM8455181 Lung, total, GEX
- GSM8455182 Ln, total, GEX
- GSM8455183 Lung, GC, GEX
- GSM8455184 Ln, GC, GEX
- GSM8455185 Lung, GC, BCR
- GSM8455186 Ln, GC, BCR
- GSE266438 Loss of Sphingosine-1-Phosphate Receptor 4 weakens innate mechanisms leading to defective lymph node hypertrophy and germinal center formation 45 samples shares germinal, center
- GSE309127 Rapid boosting increases germinal center responses to sequential vaccines 33 samples shares germinal, center
- GSE316392 Lymphocyte-derived cholinergic circuits modulate germinal center output and B cell activation 16 samples shares germinal, center
- GSE314584 Toll-Like-Receptor 5 Reduces Lung Dysbiosis and Protects Against Pulmonary Fibrosis 124 samples shares lung, like
- GSE284622 Antigen-selected gut IgA is generated from IgG1 germinal center B cells 54 samples shares germinal, center
- GSE264164 RNA-seq and ATAC-seq of follicular B cells and germinal center B cells with a conditional deletion of Brwd1 23 samples shares germinal, center
- GSE306634 DOT1L-mediated H3K79me2 directs B-cell repertoire establishment, marginal zone development and germinal center function 21 samples shares germinal, center
- GSE305877 BLIMP1 shapes germinal center B-cell clonal diversity by gating chromatin accessibility during light-to-dark zone transition 18 samples shares germinal, center
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.