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Single-nucleus RNA sequencing of human subcutaneous adipose tissue biopsies from 68 Finnish participants.

GSE274778 Homo sapiens (human) Single-nucleus RNA-seq 68 samples Submitted 2024/11/06 Platform GPL24676
At a glance

Single-nucleus RNA-seq · human · 68 samples

What it is
Single-nucleus RNA-seqGene activity measured in individual nuclei (used when whole cells are hard to isolate, for example from frozen tissue).
Organism
Homo sapiens (human)
Samples
68 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
An abdominal obesity missense variant in the adipocyte thermogenesis gene TBX15 is implicated in adaptation to cold in FinnsAmerican journal of human genetics · 2024
Summary (written by the submitters)
We performed single-nucleus RNA-seq on subcutaneous adipose tissue biopsies of 68 participants from the Roux-en-Y versus one-anastomosis gastric bypass (RYSA) cohort.
Published in
An abdominal obesity missense variant in the adipocyte thermogenesis gene TBX15 is implicated in adaptation to cold in Finns
Deal M, Kar A, Lee SHT et al. · American journal of human genetics 2024 · PMID 39515300 · doi:10.1016/j.ajhg.2024.10.001
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE274778_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1148019. Searching any of these in the dataset finder brings you back here.

Study design
A cohort of 68 individual samples

The grouping that defines any comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Sample titles run SAT00100 … SAT00199 and carry no group information.

Samples in this study

+ 28 more — browse all 68 samples with per-sample file links →

Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.