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RNA sequencing analysis of human embryonic stem cell line BG01 treated with anti-MFG-e8 neutralizing antibodies (anti-M8nAb)

GSE276921 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/04/02 Platform GPL18460
Summary
To understand the function of MFG-e8, which is secreted from human embryonic stem cells (hESCs), hESC line BG01 cells were treated with anti-M8nAb to deplete the MFG-e8 activity. Depletion of MFG-e8 activity in hESC cultures resulted in decreased cell proliferation, increased cell death, and prominent morphological changes within 2 days. To investigate the transcriptomic changes under depletion of MFG-e8 activity in hESC culture, we performed RNA-seq analysis of BG01 cells treated with anti-M8nAb at two different time point. As a result, we identified differentially expressed genes in anti-M8nAb treated BG01 cells compared to isotype IgG treated control cells. Transcriptome of anti-M8nAb tretaed BG01 cells were enriched with the genesets related with cell death, cell cycle arrest, and epithelial-to-mesenchymal transition compared to isotype IgG treated control cells. In conclusion, these RNA-seq analysis results can provide insights into how MFG-e8 affect signaling mechanisms in hESCs.
Published in
MFGE-8, a Corona Protein on Extracellular Vesicles, Mediates Self-Renewal and Survival of Human Pluripotent Stem Cells
Lee Y, Kim H, Yoon H et al. · Journal of extracellular vesicles 2025 · PMID 40130745 · doi:10.1002/jev2.70056
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Also filed as BioProject PRJNA1159727 and SRA study SRP532032. Searching any of these in the dataset finder brings you back here.

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