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Effect of 3D cultivation of human mesenchymal stomal cells to emulate the bone marrow niche microenvironment

GSE277163 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/01/15 Platform GPL34284Platform GPL15520
Summary
The bone marrow provides the niche microenvironment for the long-term maintenance of various cell types, such as hematopoietic stem cells (HSC) or long-lived plasma cells (LLPC). The maintenance of HSC and LLPC relies on specific niches composed of specific soluble molecules, adhesion molecules, and proteins of the extracellular matrix, which are partly provided by mesenchymal stromal cells. In recent years, there have been many efforts to simulate the niche situation in vitro by the generation of organ and tissue surrogates by organoid technology. In this study, we cultured bone marrow-derived human mesenchymal stromal cells on a 3D scaffold to emulate the niche microenvironment by generating a bone marrow organoid. Transcriptional analysis of differentially expressed genes and the general expression pattern of bone marrow-relevant genes in 3D demonstrate the eligibility of the 3D culture system for the maintenance of bone marrow-associated HSC and LLPC.
Published in
Survival of Human Bone Marrow Plasma Cells In Vitro Depends on the Support of the Stromal Cells, PI3K, and Canonical NF-kappaB Signaling
Uyar-Aydin Z, Kadler S, Lauster R et al. · European journal of immunology 2025 · PMID 39777683 · doi:10.1002/eji.202451358
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Also filed as BioProject PRJNA1160888 and SRA study SRP532572. Searching any of these in the dataset finder brings you back here.

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