GEO series

Obesity facilitated colon cancer progression is mediated by increased diacylglycerol o-acyltransferases 1 and 2 (DGAT1/2) levels [RNA-seq]

GSE278785 Homo sapiens (human) RNA-seq (gene expression) 26 samples Submitted 2024/10/14 Platform GPL30172Platform GPL30173

At a glance

RNA-seq (gene expression) · human · 26 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Topic: Colorectal cancerThis series is part of our curated briefing on it.
Samples: 26 samples: 8 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Obesity-Facilitated Colon Cancer Progression Is Mediated by Increased Diacylglycerol O-Acyltransferases 1 and 2 LevelsGastroenterology · 2025

Summary (written by the submitters)

Background & Aims: The obesity epidemic is associated with increased colon cancer progression. As lipid droplets (LDs) fuel tumor growth, we aim to determine the significance of diacyltransferases, DGAT1/2, responsible for LDs biogenesis, in obesity-mediated colonic tumorigenesis. Methods: Human colon cancer samples, colon cancer cells, colonospheres, and ApcMin/+ colon cancer mouse model on a high-fat diet were employed.

Read the full summary ↓

For DGAT1/2 inhibition, enzymatic inhibitors and siRNA were used. Expression, pathways, cell cycle, and growth were assessed. Bioinformatic analysis of CUT&RUN and RNAseq data was performed. Results: DGAT1/2 levels in human colon cancer tissue are significantly elevated with disease severity and obesity (vs normal). Their levels are increased in human colon cancer cells (vs non-transformed) and further enhanced by fatty acids prevalent in obesity; augmented DGAT2 expression is MYC-dependent. Inhibition of DGAT1/2 improves FOXO3 activity by attenuating PI3K, resulting in reduced MYC-dependent DGAT2 expression and LDs accumulation, suggesting feedback. This inhibition attenuated growth in colon cancer cells and colonospheres via FOXO3/p27kip1 cell cycle arrest and reduced colonic tumors in ApcMin/+ mice on a high-fat diet. Transcriptomic analysis revealed that DGAT1/2 inhibition targeted metabolic and tumorigenic pathways in human colon cancer and colon cancer crypts, stratifying human colon cancer samples from normal. Further analysis revealed that this inhibition is predictive of advanced disease-free state and survival in colon cancer patients. Conclusion: This is a novel mechanism of DGAT1/2-dependent metabolic and tumorigenic remodeling in obesity-facilitated colon cancer, which provides a platform for the future development of effective treatments for colon cancer patients.

Published in

Obesity-Facilitated Colon Cancer Progression Is Mediated by Increased Diacylglycerol O-Acyltransferases 1 and 2 Levels

Ghimire J, Collins ME, Snarski P et al. · Gastroenterology 2025 · PMID 39299402 · doi:10.1053/j.gastro.2024.09.011

This dataset

Open GSE278785 in finder → Find more RNA-seq datasets → Colorectal cancer landscape →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE278785_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1168928 and SRA study SRP536617. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 39299402) ↗

Study design

8 conditions, mostly in triplicate

HT29 DGAT1/2 Inhs ×5 HT29 Control ×3 HT29 Colonospheres Control ×3 HT29 Colonospheres DGAT1/2 Inhs ×3 APC (min/+) mouse HFD Control ×3 APC (min/+) mouse HFD DGAT1/2 Inhs ×3 APC (min/+) mouse RD Control ×3 APC (min/+) mouse RD DGAT1/2 Inhs ×3

Supports a between-group comparison across 26 samples.

How we read this: 8 replicated groups read from 26 sample titles; they account for 26 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8554827 HT29 Control 1 HT29 Control
GSM8554828 HT29 Control 2 HT29 Control
GSM8554829 HT29 Control 3 HT29 Control
GSM8554830 HT29 DGAT1/2 Inhs. 1 HT29 DGAT1/2 Inhs
GSM8554831 HT29 DGAT1/2 Inhs. 2 HT29 DGAT1/2 Inhs
GSM8554832 HT29 DGAT1/2 Inhs. 3 HT29 DGAT1/2 Inhs
GSM8554833 HT29 DGAT1/2 Inhs. 4 HT29 DGAT1/2 Inhs
GSM8554834 HT29 DGAT1/2 Inhs. 5 HT29 DGAT1/2 Inhs
GSM8554835 HT29 Colonospheres Control 1 HT29 Colonospheres Cont…
GSM8554836 HT29 Colonospheres Control 2 HT29 Colonospheres Cont…
GSM8554837 HT29 Colonospheres Control 3 HT29 Colonospheres Cont…
GSM8554838 HT29 Colonospheres DGAT1/2 Inhs. 1 HT29 Colonospheres DGAT…
GSM8554839 HT29 Colonospheres DGAT1/2 Inhs. 2 HT29 Colonospheres DGAT…
GSM8554840 HT29 Colonospheres DGAT1/2 Inhs. 3 HT29 Colonospheres DGAT…
GSM8554841 APC (min/+) mouse HFD Control 1 APC (min/+) mouse HFD C…
GSM8554842 APC (min/+) mouse HFD Control 2 APC (min/+) mouse HFD C…
GSM8554843 APC (min/+) mouse HFD Control 3 APC (min/+) mouse HFD C…
GSM8554844 APC (min/+) mouse HFD DGAT1/2 Inhs. 1 APC (min/+) mouse HFD D…
GSM8554845 APC (min/+) mouse HFD DGAT1/2 Inhs. 2 APC (min/+) mouse HFD D…
GSM8554846 APC (min/+) mouse HFD DGAT1/2 Inhs. 3 APC (min/+) mouse HFD D…
GSM8554847 APC (min/+) mouse RD Control 1 APC (min/+) mouse RD Co…
GSM8554848 APC (min/+) mouse RD Control 2 APC (min/+) mouse RD Co…
GSM8554849 APC (min/+) mouse RD Control 3 APC (min/+) mouse RD Co…
GSM8554850 APC (min/+) mouse RD DGAT1/2 Inhs. 1 APC (min/+) mouse RD DG…
GSM8554851 APC (min/+) mouse RD DGAT1/2 Inhs. 2 APC (min/+) mouse RD DG…
GSM8554852 APC (min/+) mouse RD DGAT1/2 Inhs. 3 APC (min/+) mouse RD DG…

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Search all RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.