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Placental cytotrophoblast microvillar stabilization is required for cell-cell fusion

GSE279087 Homo sapiens (human) RNA-seq (gene expression) 20 samples Submitted 2025/03/26 Platform GPL34284
At a glance

RNA-seq (gene expression) · human · 20 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
20 samples: 8 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Placental cytotrophoblast microvillar stabilization is required for cell-cell fusionDevelopment (Cambridge, England) · 2025
Summary (written by the submitters)
The placenta is an essential organ of pregnancy required for maternal-fetal transport and communication. The surface of the placenta facing the maternal blood is formed by a single giant multinucleate cell: the syncytiotrophoblast. The syncytiotrophoblast is formed and maintained via fusion of progenitor cytotrophoblasts. Cell-cell fusion is a tightly regulated process, and in non-trophoblastic cells is accompanied by stereotypical alterations in cell shape by cells that have attained fusion-competence.
Read the full summary ↓
The most prominent feature is the formation of actin-based membrane protrusions, but whether stereotypic morphological changes occur in fusion-competent cytotrophoblasts has not been characterized. Using a human placental explant model and trophoblast organoids, we identify apical microvilliation as an obligate morphological feature of fusion-competent cytotrophoblasts. Disruption of microvilli using an inhibitor of the actin-membrane cross linker protein ezrin prevented cytotrophoblast fusion and differentiation. We provide evidence that these polarized apical microvillar domains function to regulate polarized endocytosis and to spatially localize and accumulate a key glycoprotein for fusion, CD98. Thus, we propose that the polarized assembly of microvillar domains is critical for mediating efficient syncytiotrophoblast development.
Published in
Placental cytotrophoblast microvillar stabilization is required for cell-cell fusion
Duan WK, Shaha SZ, Garcia Rivas JF et al. · Development (Cambridge, England) 2025 · PMID 40213950 · doi:10.1242/dev.204619
This dataset
Open GSE279087 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE279087_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1170338 and SRA study SRP537202. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40213950) ↗
Study design
8 conditions, mostly in triplicate
Placenta, 24 hrs, ×3 Placenta, 72 hrs, ×3 Placenta, CTL, ×3 Placenta, Ezrin inhibitor, ×3 TSC, Pre-rotation, ×2 TSC, 18 hr rotation, ×2 TSC, 48 hr rotation, ×2 TSC, Ezrin inhibitor, 48 hr, ×2

Supports a between-group comparison across 20 samples.

How we read this: 8 replicated groups read from 20 sample titles; they account for 20 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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