Transcriptomic analysis of the impact of a short-term leucine deprivation and evaluation of GCN2 involvement in induced gene differential expression in human primary T cells
RNA-seq (gene expression) · human · 16 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 16 samples: 16 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Transcriptomic analysis of human primary T cells after short-term leucine-deprivation and evaluation of kinase GCN2's role in regulating differential gene expressionPloS one · 2025
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE279263_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1171747 and SRA study SRP537920. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 16 sample titles: 16 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8565519 Control 1 - DMSO
- GSM8565520 Control 2 - DMSO
- GSM8565521 Control 3 - DMSO
- GSM8565522 Control 4 - DMSO
- GSM8565523 -Leucine 1 - DMSO
- GSM8565524 -Leucine 2 - DMSO
- GSM8565525 -Leucine 3 - DMSO
- GSM8565526 -Leucine 4 - DMSO
- GSM8565527 Control 1 - GCN2 Inhibitor
- GSM8565528 Control 2 - GCN2 Inhibitor
- GSM8565529 Control 3 - GCN2 Inhibitor
- GSM8565530 Control 4 - GCN2 Inhibitor
- GSM8565531 -Leucine 1 - GCN2 Inhibitor
- GSM8565532 -Leucine 2 - GCN2 Inhibitor
- GSM8565533 -Leucine 3 - GCN2 Inhibitor
- GSM8565534 -Leucine 4 - GCN2 Inhibitor
- GSE240377 Distinct functions of wild-type and mutant Δ133p53α R273H differentially regulate glioblastoma aggressiveness and therapy-induced senescence 72 samples shares differential, induced
- GSE246920 Differential gene expression induced by combined docetaxel and darolutamide in prostate cancer xenografts 63 samples shares differential, induced
- GSE276695 Chemotherapeutic agents and leucine deprivation induce codon-biased aberrant protein production in cancer 24 samples shares deprivation, leucine
- GSE246354 GCN2 is a determinant of the response to WEE1 kinase inhibition in small-cell lung cancer 18 samples shares gcn2
- GSE260934 Coordination between the eIF2 kinase GCN2 and p53 signaling supports purine metabolism and the progression of prostate cancer 16 samples shares gcn2
- GSE301995 The tyrosine kinase inhibitor GNF-7 targets senescent cells through allosteric activation of GCN2 15 samples shares gcn2
- GSE291029 WEE1 inhibition triggers GCN2-mediated activation of the integrated stress response 12 samples shares gcn2
- GSE290313 Differentially expressed transcripts associated with depressive symptoms during pregnancy and postpartum 301 samples shares differential
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.