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The cellular response to the deprivation of L-phenylalanine from the medium in the cultivation of A375, a melanoma cell line.

GSE279290 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/12/31 Platform GPL24676
Summary
To optimize the efficacy of boron neutron capture therapy (BNCT), we investigated the optimal conditions for maximizing the uptake of boronophenylalanine (BPA), an analog of L-phenylalanine (L-Phe), in tumor cells. In the cultivation of tumor cell lines, some cells showed an amplified accumulation of BPA under 24 hours of L-Phe deprivation, which resulted in an enhancement of BNCT efficiency. In contrast, A375 demonstrated unaltered responsiveness in the uptake of BPA under L-Phe restriction. Accordingly, we conducted a comprehensive analysis of the cellular response of A375 cells under L-Phe restriction by RNA-seq.
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Also filed as BioProject PRJNA1170543 and SRA study SRP537332. Searching any of these in the dataset finder brings you back here.

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