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Deep phenotyping of peripheral mononuclear blood cells in health and disease

GSE280319 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/01/06 Platform GPL18573
Summary
Peripheral blood mononuclear cells (PBMCs) were isolated from healthy controls and patients with various diseases.. Cells were stained with CD45 and sample tags for identification purposes. CD45+ live cells were enriched through flow sorting and samples were pooled. Samples were then stained with 30 AbSeq antibodies and loaded onto 4 cartridges to account for interplate differences. Pooled sequencing libraries were then sequenced on NovaSeq 6000 (Illumina) using a S2 Reagent Kit v1.5 (200 cycles).
Published in
Deep phenotyping of T regulatory cells in psoriatic arthritis highlights targetable mechanisms of disease
McTaggart T, Lim JX, Smith KJ et al. · The Journal of biological chemistry 2025 · PMID 39662827 · doi:10.1016/j.jbc.2024.108059
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Direct links to NCBI, no account and no request form: the whole study as GSE280319_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1177839 and SRA study SRP542206. Searching any of these in the dataset finder brings you back here.

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