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Expression data from cultured mouse spermatogonia cryopreserved on the International Space Station (ISS) or on Earth

GSE280403 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/08/15 Platform GPL30172
Summary
Spermatogonial stem cells (SSCs) undergo self-renewal division to maintain spermatogenesis. In this study, we used SSCs for long-term cryopreservation in space flight. We derived germline stem cell (GS) cultures from DBA/2 mouse testes by supplementing GDNF and FGF2 (Kanatsu-Shinohara et al., 2003, Biol. Reprod. 69, 216-616). GS cells were cryopreserved in the ISS for 6 months. Frozen GS cells were thawed upon return to Earth and their gene expression profiles were analysed.
Published in
Germline transmission of cryopreserved mouse spermatogonial stem cells maintained on the International Space Station
Kanatsu-Shinohara M, Yamamoto T, Shiromoto Y et al. · Stem cell reports 2025 · PMID 40816273 · doi:10.1016/j.stemcr.2025.102602
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Also filed as BioProject PRJNA1178319 and SRA study SRP541460. Searching any of these in the dataset finder brings you back here.

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