GEO series

Macrophage peroxisomes guide alveolar regeneration and limit post-acute sequelae of SARS-CoV-2 infection (PASC)

GSE280545 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/03/10 Platform GPL34290

Summary

Peroxisomes are essential but often overlooked metabolic organelles. To explore how macrophage peroxisomes protect the lung, we conducted single-cell RNA sequencing (scRNA-seq) on lungs from both naïve and SARS-CoV-2-infected Pex5^flox/flox (WT) and Cd11c-cre Pex5^flox/flox (KO) mice at 7 days post-infection (d.p.i). scRNA-seq revealed an increased proportion of inflammatory monocytes, neutrophils, and monocyte-derived macrophages (MDMs) in the lungs of infected KO mice, alongside a marked reduction in alveolar type 2 (AT2) cells and alveolar macrophages (AMs) compared to infected WT mice. AMs from KO mice showed elevated expression of genes involved in inflammatory responses, innate immunity, and cellular stress signaling, while genes related to wound healing, cell differentiation, and lipid metabolism were downregulated, compared to AMs from WT mice at 7 d.p.i.

Published in

Macrophage peroxisomes guide alveolar regeneration and limit SARS-CoV-2 tissue sequelae

Wei X, Qian W, Narasimhan H et al. · Science (New York, N.Y.) 2025 · PMID 40048515 · doi:10.1126/science.adq2509

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Direct links to NCBI, no account and no request form: the whole study as GSE280545_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1179376 and SRA study SRP541818. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40048515) ↗

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