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TSA-sequencing in untreated (UN), sodium arsenite (SA) and heat shock (HS) stressed HeLa cells

GSE280712 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2025/05/26 Platform GPL24676
Summary
Mammalian cells activate diverse defense mechanisms in response to stresses. Nuclear stress bodies (nSBs) are transient membraneless organelles in primates that only form upon severe stresses. Little is known on how their formation contributes to cellular homeostasis or whether nSBs have any pathophysiological roles. We observed that the de novo formation of nSBs accompanies enlarged pericentromeric SatⅢ DNA chromatin loci, prompting us to investigate whether such an acute alteration impacts regional chromatin accessibility and gene expression. To test this, we performed an improved Tyramide Signal Amplification (TSA) mapping to identify genes proximal to newly formed nSBs.
Published in
De novo assembly of nuclear stress bodies rearranges and enhances NFIL3 to restrain acute inflammatory responses
Liu XQ, Li P, Gao BQ et al. · Cell 2025 · PMID 40436014 · doi:10.1016/j.cell.2025.05.003
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Also filed as BioProject PRJNA1180084 and SRA study SRP542201. Searching any of these in the dataset finder brings you back here.

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