GEO series

Mesenchymal IL-33 licenses reparative macrophages to facilitate endovascular injury-induced neointimal hyperplasia [scRNA-Seq]

GSE281009 Mus musculus (mouse) Single-cell RNA-seq 3 samples Submitted 2025/11/27 Platform GPL24247

At a glance

Single-cell RNA-seq · mouse · 3 samples

What it is: Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism: Mus musculus (mouse)
Samples: 3 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Perivascular mesenchymal cells instruct ST2+ reparative macrophages to promote endovascular injury-induced neointimal hyperplasia in miceNature communications · 2026

Summary (written by the submitters)

Immune remodeling is crucial for tissue repair, but the interaction between arterial macrophages and mesenchymal stromal cells (MSCs) in neointimal hyperplasia is not well understood. Herein, we identified Il33-expressing MSCs serve as the key regulators of arterial immune responses following endovascular injury via facilitating the recruitment of reparative macrophages.

Read the full summary ↓

Using temporal-resolving scRNA-seq and multi-color flow cytometry, we revealed a distinct subset of LY6C-CD63+CD72+ reparative macrophages essential for vascular smooth muscle cell (VSMC) proliferation and neointimal formation. These ST2-expressing macrophages are recruited and activated by MSC-derived IL-33, which is upregulated via NFκB signaling following injury, and in turn amplify the osteopontin (OPN) production that drives VSMC proliferation. Furthermore, hydrogel-mediated local delivery of siRNAs targeting Il33 or Spp1 reduced neointimal hyperplasia, confirming the importance of the IL-33-ST2-OPN axis in pathological vascular remodeling. Our findings uncover a critical role of mesenchymal IL-33 in orchestrating immune cell recruitment and promoting VSMC proliferation, suggesting that targeting this pathway may offer therapeutic potential for preventing restenosis and other vascular diseases.

Published in

Perivascular mesenchymal cells instruct ST2+ reparative macrophages to promote endovascular injury-induced neointimal hyperplasia in mice

Ping Y, Qin Z, Huang X et al. · Nature communications 2026 · PMID 41794821 · doi:10.1038/s41467-026-68587-x

This dataset

Open GSE281009 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE281009_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1181826 and SRA study SRP543057. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41794821) ↗

Samples in this study

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+ 3 more — browse all 3 samples with per-sample file links →

Datasets on a similar topic

GSE281008 Mesenchymal IL-33 licenses reparative macrophages to facilitate endovascular injury-induced neointimal hyperplasia [RNA-Seq] 7 samples shares il-33, injury-induced, endovascular, mesenchymal, macrophages, hyperplasia, reparative, facilitate
GSE268774 Lymph node macrophages drive immune tolerance and resistance to cancer therapy by expression of the immune-regulatory cytokine IL-33 [RNA-seq] 48 samples shares il-33, macrophages
GSE226344 Primary lung fibroblasts respond to IL-33, IL-13, and IL-17a and secrete factors that activate macrophages 39 samples shares il-33, macrophages
GSE226343 Primary lung fibroblasts respond to IL-33, IL-13, and IL-17a and secrete factors that activate macrophages [JV214] 24 samples shares il-33, macrophages
GSE268778 Lymph node macrophages drive immune tolerance and resistance to cancer therapy by expression of the immune-regulatory cytokine IL-33 [scRNA-seq] 20 samples shares il-33, macrophages
GSE226342 Primary lung fibroblasts respond to IL-33, IL-13, and IL-17a and secrete factors that activate macrophages [JV210] 15 samples shares il-33, macrophages
GSE273883 CX3CR1+ Synovial Macrophages for Barrier Restore in Rheumatoid Arthritis: A New Target for Mesenchymal Stem Cell-Based Therapy? 35 samples shares mesenchymal, macrophages
GSE263616 Heparan sulfate regulates amphiregulin programming of tissue reparative lung mesenchymal cells during influenza A virus infection in mice 24 samples shares mesenchymal, reparative

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