FFPE-ATAC and PDX-ATAC sequence data of small cell lung cancer

Small cell lung cancer (SCLC) is known for its high metastatic potential, with most patients demonstrating clinically evident metastases in multiple organs at diagnosis. The factors contributing to this exceptional metastatic capacity have not been defined. To bridge this gap, we compared gene expression in SCLC patient samples who never experienced metastasis or relapse throughout their clinical course, versus primary SCLC patient samples from more typical patients who had metastatic disease at diagnosis. This analysis identified FOXA2 as a transcription factor strongly associated with SCLC metastasis. To clarify the contribution of ASCL1 with the interaction with FOXA2, we performed ATAC-seq on clinical FFPE samples, and PDX samples to analyze the chromatin accessibility in FOXA2+ and FOXA2- state. Furthermore, we confirmed the binding of ASCL1 by ChiP-sequence.