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Single-cell RNA sequencing of human IL-18R supported CAR T cells targeting oncofetal Tenascin C

GSE282046 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2024/11/20 Platform GPL34284
Summary
C.TNC-CAR.Zip18R CAR-T cell of 2 donors were used for 2 in vitro experiments. In the first experiment, we did scRNA-seq for activated CAR-T cells. In the second experiment, the CAR-T cells of each donor were cocultured with tumor cells for 12 hrs, 24 hrs and 48 hrs. The CAR-T cells for each timepoints were extracted for scRNA-seq. Multiplexed 10x Genomics scRNA-seq were performed with a hashtag to label each sample.
Published in
IL-18R supported CAR T cells targeting oncofetal tenascin C for the immunotherapy of pediatric sarcoma and brain tumors
Wickman E, Lange S, Wagner J et al. · Journal for immunotherapy of cancer 2024 · PMID 39572158 · doi:10.1136/jitc-2024-009743
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Direct links to NCBI, no account and no request form: the whole study as GSE282046_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1186711. Searching any of these in the dataset finder brings you back here.

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