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Spatial confinement induces reciprocating migration of epidermal keratinocytes and forms triphasic epithelia

GSE282553 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/02/18 Platform GPL24676
Summary
Epithelial cells undergo epithelial-mesenchymal transition (EMT) during migration and regain their epithelial phenotype in the post-migration phase (mesenchymal-epithelial transition; MET). Keratinocytes (KCs), skin epithelial cells, placed on a microporous membrane migrated through 3.0 µm or larger micropores. Live imaging of immortalized HaCaT KCs which stably express green fluorescent protein (GFP)-labelled LifeAct (LifeAct-HaCaTs) revealed that KCs moved in a reciprocating manner with actin-rich filopodia-like structures of KCs extending into and out of the 3.0 µm micropores. HaCaT KCs cultured on 3.0 µm pored-membranes upregurated the genes associated with tissue development and cell differenciation compared with on 0.4 µm-pored membranes. These results demonstrate that KCs migrate through confined spaces in a reciprocating manner, which might help form triphasic epithelia, recapitulating wound healing processes.
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Also filed as BioProject PRJNA1189126 and SRA study SRP546819. Searching any of these in the dataset finder brings you back here.

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