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Chromatin binding of nuclear transport factor Importin α in human cultured cancer cells

GSE283000 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 3 samples Submitted 2026/04/05 Platform GPL18460
Summary
Micronuclei (MN) are membrane-enclosed structures that contain DNA or chromatin, and their presence can lead to genomic instability. We found that importin α, a key nuclear transport factor, is highly concentrated in the MN in cultured human cancer cells. Notably, importin α is not uniformly distributed across all MN; it is localized to approximately 40% of them. This selective localization is characterized by an uncorrelated distribution relative to importin β1, CAS/CSE1L, and Ran, suggesting uncontrolled its nuclear-cytoplasmic recycling. Additionally, along with an association with euchromatin regions, we identified chromatin-regulating molecules as potential interactors with importin α and noted a significant reduction in the mobility of importin α within MN, indicating the establishment of a unique microenvironment in these importin α-enriched structures. These results indicate that importin α serves as a previously unrecognized molecular marker for assessing the quality control of MN in human cancer cells.
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Also filed as BioProject PRJNA1191433 and SRA study SRP547984. Searching any of these in the dataset finder brings you back here.

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