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Symbiotic Adhesion of Neutrophils and Epithelial-like Cancer Cells Shapes the Tumor Microenvironment and Promotes Metastasis [1] [coculture]

GSE284277 Mus musculus (mouse) RNA-seq (gene expression) 9 samples Submitted 2026/05/21 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 9 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
9 samples: 3 × Bone marrow neutrophils cultured in 0.5% FBS for 12 hours vs 3 × Sorted bone marrow neutrophils after cocultured with PyMT-M cells in DMEM with 0.5% FBS for 12 hours vs 3 × Sorted bone marrow neutrophils after cocultured with PyMT-N cells in DMEM with 0.5% FBS for 12 hoursGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
ICAM1high Neutrophils Sculpt Tumor Evolution and Metastasis Through Symbiotic Adhesion and Reverse MigrationCancer research · 2026
Summary (written by the submitters)
Neutrophils play complicated roles in tumor progression. Examination of several murine triple negative breast cancer (TNBC) models revealed nearly opposite impact of neutrophils on epithelial-like vs. mesenchymal-like cancer cells (ECCs vs. MCCs). Interaction with ECCs upregulates ICAM1 in neutrophils, a feature that also distinguishes tumor-infiltrating neutrophils from other neutrophils in human.
Read the full summary ↓
Direct adhesion between ECCs and neutrophils, especially the ICAMhigh subpopulation, promote the survival of each other – forming a symbiotic relationship. In contrast, MCCs are thwarted by neutrophils due to decreased cell adhesion and elastase resistance. The ICAM1high neutrophils are known for reverse migration to circulation. The adhesive and reverse migratory properties of ICAM1high neutrophils mediate intravasation of metastatic seeds. These observations were verified in a subset of human TNBCs that unexpectedly enrich non-Hispanic European patients. Thus, we demonstrated a co-evolution through which neutrophils sculpt phenotypes and metastatic behaviors of TNBC, which may preferentially occur in patients of certain race/ethnicity.
Published in
ICAM1high Neutrophils Sculpt Tumor Evolution and Metastasis Through Symbiotic Adhesion and Reverse Migration
Wu L, Xu Z, Wang J et al. · Cancer research 2026 · PMID 42207963 · doi:10.1158/0008-5472.CAN-25-3935
This dataset
Open GSE284277 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE284277_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1198326 and SRA study SRP551446. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42207963) ↗
Study design
3 × Bone marrow neutrophils cultured in 0.5% FBS for 12 hours vs 3 × Sorted bone marrow neutrophils after cocultured with PyMT-M cells in DMEM with 0.5% FBS for 12 hours vs 3 × Sorted bone marrow neutrophils after cocultured with PyMT-N cells in DMEM with 0.5% FBS for 12 hours

Supports a between-group comparison across 9 samples.

How we read this: 3 replicated groups read from 9 sample titles; they account for 9 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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