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Lung tissue-resident memory T cells optimize protection by IL-10 regulation of innate immunity

GSE284691 Mus musculus (mouse) RNA-seq (gene expression) 43 samples Submitted 2025/07/01 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 43 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
43 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Lung tissue-resident memory T cells optimize protection by IL-10 regulation of innate immunityThe Journal of experimental medicine · 2026
Summary (written by the submitters)
Respiratory viral infections establish tissue resident memory T (TRM) cells in the lung, which provide optimal protection against infectious challenge, though the underlying mechanisms remain incompletely understood. Here, using a mouse model of heterosubtypic influenza infection, we demonstrate that the lung-localized secondary immune response exhibits an attenuated innate immune response compared to the primary response due to TRM-mediated production of the immunoregulatory cytokine IL-10.
Read the full summary ↓
Using reporter mice, we identify that lung TRM are the predominant producers of early IL-10 in response to viral challenge. Inhibition of early IL-10 signaling in secondary infections results in increased morbidity and lung pathology, and reveals pleiotropic effects of IL-10 in regulating macrophage inflammation and enhancing IFN-g production by memory CD8+T cells. Human influenza-specific TRM isolated from lungs recapitulate robust IL-10 expression associated with augmented effector responses of murine TRM. These data support a dual role for TRM in coordinating in situ secondary responses - augmenting effector responses for robust viral clearance while dampening inflammation to limit tissue damage.
Published in
Lung tissue-resident memory T cells optimize protection by IL-10 regulation of innate immunity
Yang AY, Davis-Porada J, Paik DH et al. · The Journal of experimental medicine 2026 · PMID 41081716 · doi:10.1084/jem.20242307
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE284691_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1200051 and SRA study SRP552368. Searching any of these in the dataset finder brings you back here.

Samples in this study

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