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Single-nuclei RNA-seq of neural organoids generated from wildtype and FNDC4 KO iPSCs

GSE285126 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/06/16 Platform GPL34284
Summary
Large-cohort genome-wide association studies (GWAS) for alcohol use disorder (AUD) risk and drug treatment outcomes have identified significant genetic loci that are splicing quantitative trait loci (sQTLs) for the FNDC4 gene in multiple human brain regions. However, in spite of the fact that FNDC4 is highly expressed in the brain, its function and how it might contribute to AUD pathophysiology remain unknown. In the precent study, we characterized FNDC4 function using CRISPR/cas9 gene editing, the creation of human induced pluripotent stem cell (iPSC)-derived neural organoids and with single-nucleus RNA sequencing (snRNA-seq).
Published in
Alcohol Use Disorder Associated Gene FNDC4 Alters Glutamatergic and GABAergic Neurogenesis
Zhu X, John AJ, Wang L et al. · bioRxiv : the preprint server for biology 2025 · PMID 40463052 · doi:10.1101/2025.05.15.654319
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Also filed as BioProject PRJNA1201214 and SRA study SRP553052. Searching any of these in the dataset finder brings you back here.

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