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Endothelial SRSF1 directly regulates ATF3-KLF2 pathway to promote ischemia-induced angiogenesis [ALP_RNA-seq]

GSE285367 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/10/27 Platform GPL24676
Summary
Mechanistically, SRSF1 modulated ATF3 alternative splicing, wherein SRSF1 directly bound to ATF3 pre-mRNA and elevated full-length ATF3 transcript at the expense of truncated ATF3Δzip2 transcript, and in turn, ATF3 directly bound to the KLF2 promoter and suppressed KLF2-S1PR1 signaling, through which mediated the SRSF1-promoted endothelial tube formation and angiogenesis. Additionally, alprostadil, the prostaglandin E1 analog, could activate the SRSF1 signaling to improve endothelial angiogenesis in vitro and in vivo. Our findings identified SRSF1 as a novel regulator of ischemia-induced angiogenesis that enhances endothelial angiogenic functions by regulating the ATF3-KLF2/SIPR1 pathway. These results suggest that modulation of endothelial SRSF1 may represent a promising therapeutic approach for treating ischemic vascular diseases.
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Also filed as BioProject PRJNA1203235 and SRA study SRP553917. Searching any of these in the dataset finder brings you back here.

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