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Long-term maintenanance of patient-specific characteristics in tumoroids from six cancer indications

GSE285457 Homo sapiens Expression profiling by high throughput sequencing 5 samples Submitted 2025/01/31 Platform GPL24676
Summary
Tumoroids, sometimes referred to as cancer organoids, are patient-derived cancer cells grown as 3D, self-organized multicellular structures that maintain key characteristics (e.g., genotype, gene expression levels) of the tumors from which they originated. These models have emerged as valuable tools for studying tumor biology, cytotoxicity, and the response of patient-derived cells to cancer therapies. However, the establishment and maintenance of tumoroids have historically been challenging, labor-intensive, and highly variable from lab to lab, hindering their widespread use. Here, we established patient-derived colorectal cancer tumoroid lines in OncoPro Tumoroid Culture Medium and performed RNA profiling of single cells from primary dissociated tumors and the corresponding tumoroids to compare the transcriptomic concordance in primary and OncoPro-derived samples.
Published in
Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
Paul CD, Yankaskas C, Shahi Thakuri P et al. · Scientific reports 2025 · PMID 39890889 · doi:10.1038/s41598-025-86979-9
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Direct links to NCBI, no account and no request form: the whole study as GSE285457_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 5 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1203668 and SRA study SRP554432. Searching any of these in the dataset finder brings you back here.

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