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Fibroblast-Derived Thrombospondin 2 Shapes the Immune-Exclusionary Environment at Invasive Fronts in Colorectal Cancer [scRNA-seq]

GSE285504 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/10/28 Platform GPL28457
Summary
Fibrotic colorectal cancers (CRC) are characterized by immune exclusion driven by activated stroma, particularly by cancer-associated fibroblasts (CAFs). We identified that the matricellular protein thrombospondin-2 (THBS2) is highly expressed in the stroma of fibrotic CRCs. We performed orthotopic implantation of CMS4-like mouse tumor organoid (MTO) into WT and Thbs2 KO mice. Tumors developed in Thbs2 KO mice were considerably smaller and exhibited increased CD8 T cell infiltration and higher numbers of apoptotic tumor cells compared with WT mice. Our aim is to elucidate how Thbs2 in tumor microenvironment influences tumor growth.
Published in
Targeting fibroblast derived thrombospondin 2 disrupts an immune-exclusionary environment at the tumor front in colorectal cancer
Iwane K, Nakanishi Y, Muta Y et al. · Nature communications 2025 · PMID 41276505 · doi:10.1038/s41467-025-66485-2
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Also filed as BioProject PRJNA1204095 and SRA study SRP554285. Searching any of these in the dataset finder brings you back here.

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