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Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression [Cut & Tag]

GSE285915 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2025/01/11 Platform GPL34284
Summary
Anillin (ANLN), a mitotic protein that regulates contractile ring assembly, has been reported as an oncoprotein. However, the function of ANLN in cancer cells, especially in the nucleus, has not been fully understood. Here, we report a previously unknown role of nuclear ANLN in gene transcriptional regulation. We found that nuclear ANLN directly interacts with the RNA polymerase II (Pol II) large subunit. ANLN enhances initiated Pol II clustering and promotes Pol II CTD phase separation. Short-term depletion of ANLN alters the chromatin binding and enhancer-mediated transcriptional activity of Pol II. The target genes of ANLN-Pol II axis are involved in oxidoreductase activity, Wnt signaling and cell differentiation. Our results reveal the function of nuclear ANLN in transcriptional regulation, providing a theoretical basis for ESCC treatment.
Published in
Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression
Cao YF, Wang H, Sun Y et al. · Nature communications 2025 · PMID 39894879 · doi:10.1038/s41467-025-56645-9
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Also filed as BioProject PRJNA1206966 and SRA study SRP555460. Searching any of these in the dataset finder brings you back here.

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