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Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression [RNA-Seq]

GSE285916 Homo sapiens (human) RNA-seq (gene expression) 12 samples Submitted 2025/01/11 Platform GPL34284
At a glance

RNA-seq (gene expression) · human · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
12 samples: 3 × KYSE150, siNC for 24 hours, biol vs 3 × KYSE150, siANLN for 24 hours, biol vs 3 × KYSE150, siNC for 48 hours, biol vs 3 × KYSE150, siANLN for 48 hours, biolGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expressionNature communications · 2025
Summary (written by the submitters)
Anillin (ANLN), a mitotic protein that regulates contractile ring assembly, has been reported as an oncoprotein. However, the function of ANLN in cancer cells, especially in the nucleus, has not been fully understood. Here, we report a previously unknown role of nuclear ANLN in gene transcriptional regulation. We found that nuclear ANLN directly interacts with the RNA polymerase II (Pol II) large subunit.
Read the full summary ↓
ANLN enhances initiated Pol II clustering and promotes Pol II CTD phase separation. Short-term depletion of ANLN alters the chromatin binding and enhancer-mediated transcriptional activity of Pol II. The target genes of ANLN-Pol II axis are involved in oxidoreductase activity, Wnt signaling and cell differentiation. Our results reveal the function of nuclear ANLN in transcriptional regulation, providing a theoretical basis for ESCC treatment.
Published in
Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression
Cao YF, Wang H, Sun Y et al. · Nature communications 2025 · PMID 39894879 · doi:10.1038/s41467-025-56645-9
This dataset
Open GSE285916 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE285916_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1206978 and SRA study SRP555470. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 39894879) ↗
Study design
3 × KYSE150, siNC for 24 hours, biol vs 3 × KYSE150, siANLN for 24 hours, biol vs 3 × KYSE150, siNC for 48 hours, biol vs 3 × KYSE150, siANLN for 48 hours, biol

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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