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ZC3HC1 has functions distinct from TPR and is dispensable for TPR localization to the nuclear basket

GSE286436 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/02/18 Platform GPL30173
Summary
Background: The nuclear basket is a ‘fishtrap’-like structure on the nucleoplasmic face of the nuclear pore complex which has been implicated in diverse functions including RNA export, heterochromatin organisation, and mitosis. Recently, a novel component of the nuclear basket, ZC3HC1, has been described. The localisation of ZC3HC1 to nuclear pores has been reported to occur reciprocally with TPR, a major structural component of the nuclear basket. Methods and Results: Using immunofluorescence and RNA sequencing, here we show that in human fibroblasts, although ZC3HC1 localisation to nuclear pores is TPR-dependent, TPR localises to pores regardless of the presence of ZC3HC1. We demonstrate that knockdown of TPR and ZC3HC1 produce distinct transcriptional profiles. Conclusions: Our results suggest that there is little overlap in function between these two nuclear basket proteins
Published in
ZC3HC1 has many functions distinct from TPR and is dispensable for TPR localisation to the nuclear basket
M Bartlett B, Acosta JC, A Bickmore W · Wellcome open research 2025 · PMID 40443800 · doi:10.12688/wellcomeopenres.23711.2
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Also filed as BioProject PRJNA1209204 and SRA study SRP556615. Searching any of these in the dataset finder brings you back here.

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