Nitric oxide drives proteomic diversity through alternative splicing [HEK RNA-Seq]
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 12 samples: 3 × WT PTB + NOS1, biol vs 3 × C251S PTB + NOS1, biol vs 3 × WT PTB + empty vector, biol vs 3 × C251S PTB + empty vector, biolGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Nitric oxide drives proteomic diversity through alternative splicingMolecular cell · 2026
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE286544_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1209889 and SRA study SRP556952. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8729071 WT PTB + NOS1, biol rep 1 WT PTB + NOS1, biol
- GSM8729072 WT PTB + NOS1, biol rep 2 WT PTB + NOS1, biol
- GSM8729073 WT PTB + NOS1, biol rep 3 WT PTB + NOS1, biol
- GSM8729074 C251S PTB + NOS1, biol rep 1 C251S PTB + NOS1, biol
- GSM8729075 C251S PTB + NOS1, biol rep 2 C251S PTB + NOS1, biol
- GSM8729076 C251S PTB + NOS1, biol rep 3 C251S PTB + NOS1, biol
- GSM8729077 WT PTB + empty vector, biol rep 1 WT PTB + empty vector, …
- GSM8729078 WT PTB + empty vector, biol rep 2 WT PTB + empty vector, …
- GSM8729079 WT PTB + empty vector, biol rep 3 WT PTB + empty vector, …
- GSM8729080 C251S PTB + empty vector, biol rep 1 C251S PTB + empty vecto…
- GSM8729081 C251S PTB + empty vector, biol rep 2 C251S PTB + empty vecto…
- GSM8729082 C251S PTB + empty vector, biol rep 3 C251S PTB + empty vecto…
- GSE303137 HNRNPH controls alternative splicing cooperatively on a transcriptome-wide scale [RNA-seq] 36 samples shares alternative, splicing, rna-seq
- GSE330179 mRNA-seq analysis of S100A16-mediated alternative splicing in lung adenocarcinoma under oxidative stress 18 samples shares alternative, splicing, rna-seq
- GSE226054 Comprehensive mRNA-sequencing-based characterization of three HEK-293 cell lines during an rAAV production process for Gene Therapy Applications 120 samples shares hek, rna-seq
- GSE277967 Reconstitution of Human Brain Cell Diversity in Organoids via Four Protocols [time-course bulk RNA-seq] 768 samples shares diversity, rna-seq
- GSE287540 Transcriptomic and proteomic characterization of cell and protein biomarkers of checkpoint inhibitor-induced liver injury [bulk RNA-seq] 128 samples shares proteomic, rna-seq
- GSE334651 Alternative splicing- and gene fusion-derived neoepitopes in MASLD-associated hepatocellular carcinoma 120 samples shares alternative, splicing
- GSE268177 Modulating alternative splicing of MECP2: A potential therapeutic strategy for Rett syndrome 93 samples shares alternative, splicing
- GSE287541 Transcriptomic and proteomic characterization of cell and protein biomarkers of checkpoint inhibitor-induced liver injury [scRNA-seq] 92 samples shares proteomic, rna-seq
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.