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Lung B cells in ectopic germinal centres undergo affinity maturation

GSE286951 Mus musculus Expression profiling by high throughput sequencing; Other 8 samples Submitted 2025/02/27 Platform GPL34475
Summary
Chronic exposure to allergens and other airborne antigens can result in the formation of lymphocyte aggregates in the lung, which can harbour ectopic germinal centres (GCs). After allergen exposure, GCs that form in the lung are much smaller and less densely packed with B cells than lymph node GCs. Despite this, ectopic lung GCs can support somatic hypermutation and affinity-based maturation as in lymph node GCs, and can export memory B cells directly into the lung tissue. This demonstrates that the lung itself can locally diversify B cell responses and support the generation of tissue memory B cell populations in situ. Here, we have employed a single cell sequencing approach using 10x Genomics scRNAseq and BCRseq to study germinal centre B cell clonal sharing between the mediastinal lymph node and lungs of mice.
Published in
Lung B cells in ectopic germinal centers undergo affinity maturation
Guillaume SM, Foster WS, San Martín Molina I et al. · Proceedings of the National Academy of Sciences of the United States of America 2025 · PMID 40168127 · doi:10.1073/pnas.2416855122
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Also filed as BioProject PRJNA1210598 and SRA study SRP557275. Searching any of these in the dataset finder brings you back here.

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