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Effect of hypoxia on HIF-1α binding genes in SUM159 breast cancer cells with ChIP-Seq.

GSE287168 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2025/05/15 Platform GPL24676
Summary
Brain metastasis is a major cause of breast cancer mortality, but the molecular basis and pathological mechanisms are poorly understood. Here, we determined that integrin β3 (ITGB3) expression mediated by hypoxia-inducible factor 1 (HIF-1) plays a critical role in brain metastasis of breast cancer. Hypoxia induces HIF-1-dependent expression of ITGb3, which is required for breast cancer cell migration and invasion. Knockdown of HIF-1a or ITGB3 expression impairs the colonization of breast cancer cells to brain after injection into the cardiac left ventricle. Mechanistically, ITGB3 protein expressed by breast cancer cells was incorporated into extracellular vesicles (EVs). The ITGb3+ EVs associated with brain endothelial cells, activated vascular endothelial growth factor receptor 2 signaling, and increased endothelial permeability to facilitate metastatic colonization of the brain.
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Direct links to NCBI, no account and no request form: the whole study as GSE287168_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1211545 and SRA study SRP557824. Searching any of these in the dataset finder brings you back here.

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