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An integrated transcriptomic cell atlas of human endoderm-derived organoids

GSE287233 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/04/29 Platform GPL24676
Summary
Human pluripotent stem cells and tissue-resident fetal and adult stem cells can generate epithelial tissues of endodermal origin in vitro that recapitulate aspects of developing and adult human physiology. Here, we integrate single-cell transcriptomes from 218 samples covering organoids and other models of diverse endoderm-derived tissues to establish an initial version of a human endoderm-derived organoid cell atlas (HEOCA). The integration includes nearly one million cells across diverse conditions, data sources and protocols. We compare cell types and states between organoid models, and harmonize cell annotations through mapping to primary tissue counterparts. Focusing on the intestine and lung, we provide examples of mapping data from new protocols, and show how the atlas can be used as a diverse cohort to assess perturbations and disease models. The HEOCA makes diverse datasets centrally available, and will be valuable to assess fidelity, characterize perturbed and diseased states, and streamline protocol development.
Published in
An integrated transcriptomic cell atlas of human endoderm-derived organoids
Xu Q, Halle L, Hediyeh-Zadeh S et al. · Nature genetics 2025 · PMID 40355592 · doi:10.1038/s41588-025-02182-6
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Direct links to NCBI, no account and no request form: the whole study as GSE287233_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1211661. Searching any of these in the dataset finder brings you back here.

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