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Discovery, delineation and therapeutic targeting of a hyper-translation pathway driving cytokine release syndrome [BMDM_Ribo-seq]

GSE287441 Mus musculus Other; Expression profiling by high throughput sequencing 6 samples Submitted 2025/12/01 Platform GPL24247
Summary
Cytokine release syndrome (CRS) is a potentially life-threatening inflammatory condition. However, the defining features that distinguish it from self-resolving inflammation remain poorly understood. In this study, we identified monocyte/macrophage hyper-translation as a hallmark of CRS pathogenesis in patient samples. To uncover the molecular drivers of this phenomenon, a CRISPR screen followed by genetic validation pinpointed BCAP as a critical regulator of hyper-translation. Mechanistically, BCAP activated the RSK-EIF4B axis, fueling hyperactive translation in macrophages. Genetic ablation of RSK attenuated CRS-associated inflammation, and pharmacological inhibition of RSK alleviated CRS symptoms in a humanized mouse model. These findings establish hyper-translation as a key pathogenic feature of CRS and highlight protein translation as a druggable pathway, opening venues for therapeutic interventions of CRS and other inflammatory diseases.
Published in
Discovery, delineation, and therapeutic targeting of a hyper-translation pathway driving cytokine release syndrome
Liu X, Li J, Zhang Y et al. · Cell reports. Medicine 2026 · PMID 41475340 · doi:10.1016/j.xcrm.2025.102531
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Direct links to NCBI, no account and no request form: the whole study as GSE287441_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1212760 and SRA study SRP558376. Searching any of these in the dataset finder brings you back here.

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