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JAX Study 5- RNA-seq of male KOLF2.2J hiPSC-derived cortical brain organoids and extra-embryonic lineages homozygous null for nine different transcription factors and one RNA splicing factor

GSE287844 Homo sapiens Expression profiling by high throughput sequencing 48 samples 2025/02/03 GPL34281
Summary
This study aimed to phenotype iPSC-derived cortical brain organoids that contain homozygous null alleles for transcription factors SIX3 and NR2F1 and to phenotype iPSC-derived extra-embryonic cell lineages that contain homozygous null alleles for transcription factors HEY1, HOPX, MAFF, TWIST2, DLX4, DLX5, and DLX6 and the splicing factor MBNL2. Null alleles were generated by the insertion of a premature termination codon with frameshift (PTC+1). This analysis provided insight into the impact of loss of function for these transcription factors in the respective cell lineages.
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