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Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC - First round of pooled screening identified an initial set of TFs for inducing microglia gene expression.

GSE287847 Homo sapiens Expression profiling by high throughput sequencing; Other 6 samples Submitted 2025/03/23 Platform GPL30173Platform GPL15520
Summary
Differentiation of induced pluripotent stem cells (iPSCs) into specialized cell types is essential for uncovering cell-type specific molecular mechanisms and interrogating cellular function. Transcription factor (TF) screens have enabled efficient production of a few cell types; however, engineering cell types that require complex TF combinations remains challenging. Here, we report an iterative, high-throughput single-cell TF screening method that enables identification of TF combinations for specialized cell differentiation, which we validated by differentiating human microglia-like cells. We found that the expression of six TFs, SPI1, CEBPA, FLI1, MEF2C, CEBPB, and IRF8, is sufficient to differentiate human iPSC into cells with transcriptional and functional similarity to primary human microglia within 4 days.
Published in
Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC
Liu S, Li L, Zhang F et al. · Nature communications 2025 · PMID 40494892 · doi:10.1038/s41467-025-59596-3
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Also filed as BioProject PRJNA1214808 and SRA study SRP559464. Searching any of these in the dataset finder brings you back here.

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