← BioTransfer GEO Dataset Finder
GEO series

tRNA m1A modification in macrophages promotes translation of interferon pathway genes to enhance CD8+ T cell-mediated anti-tumor immunity [CAR-iMACs RNA-seq]

GSE288142 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2026/03/04 Platform GPL24676
Summary
The involvement of tRNA m1A modification in the anti-tumoral tumor-associated macrophages (TAMs) has been validated in our study that macrophage-specific knockout of m1A “writer” enzymes TRMT61A leads to increased infiltration of TAMs, impaired CD8+ T cell function, and enhanced tumor growth. Since we observed that tumor-derived components suppresses tRNA m1A modification in macrophages, we sought to identify which component in the TME induces the down-regulation of tRNA m1A modification. Here, we performed bulk RNA-seq using TRMT61AOE-CAR-iMACs and CAR-iMACs to ensure the effect of human Trmt61a overexpression on CAR-iMACs. Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that signal transduction is the most up-regulated pathway in CAR-iMACs after human Trmt61a overexpression.
Published in
tRNA m(1)A modification orchestrates STING translation in macrophages to enhance antitumor immunity and CAR-macrophage immunotherapy
Wang X, Wang X, Li H et al. · Cellular & molecular immunology 2026 · PMID 41535541 · doi:10.1038/s41423-025-01383-7
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE288142_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1216274 and SRA study SRP560231. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 4 more — browse all 4 samples with per-sample file links →

Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.