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Genome-wide mapping of H3K27ac by Cut&Tag in CD4+ and CD8+ immune cells of astronauts following prolonged orbital space flight.

GSE288469 Homo sapiens (human) ChIP-seq / ATAC-seq (genome binding and open chromatin) 36 samples Submitted 2025/09/17 Platform GPL24676
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · human · 36 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Homo sapiens (human)
Samples
36 samples: 18 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Epigenomic profiling of immune cell subtypes reveals H3K27ac-marked stress signatures after long-duration spaceflightScientific reports · 2025
Summary (written by the submitters)
Cut&Tag for H3K27ac was performed in isolated CD4+ and CD8+ immune cells of astronauts undergoing prolonged orbital space flight and in control subjects.
Published in
Epigenomic profiling of immune cell subtypes reveals H3K27ac-marked stress signatures after long-duration spaceflight
Fullstone TL, Fischer LFJ, Bohmeier M et al. · Scientific reports 2025 · PMID 40940371 · doi:10.1038/s41598-025-17930-1
This dataset
Open GSE288469 in finder → Find more human ChIP / ATAC / CUT&Tag datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE288469_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1217808 and SRA study SRP560873. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40940371) ↗
Study design
18 conditions, mostly in duplicate
A1_PF_CD ×2 A1_R+1_CD ×2 A1_R+35_CD ×2 A2_PF_CD ×2 A2_R+1_CD ×2 A2_R+35_CD ×2 A3_PF_CD ×2 A3_R+1_CD ×2 +10 more

Supports a between-group comparison across 36 samples.

How we read this: 18 replicated groups read from 36 sample titles; they account for 36 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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