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Differential oligomerization regulates PHF13 chromatin function and affinity [RNA-seq]

GSE289538 Homo sapiens (human) RNA-seq (gene expression) 18 samples Submitted 2025/05/29 Platform GPL34295
At a glance

RNA-seq (gene expression) · human · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
18 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Differential oligomerization regulates PHF13 chromatin affinity and functionNucleic acids research · 2025
Summary (written by the submitters)
PHF13 is a H3K4me3 epigenetic reader, that modulates key chromatin processes including transcription, DNA damage response and chromatin architecture. PHF13 is found aberrantly regulated in different cancers and its misexpression alters the epigenetic landscape of key transcription factors that regulate Epithelial to Mesenchymal transition.
Read the full summary ↓
In this study we sought to understand how PHF13’s chromatin affinity and diverse chromatin functions are intrinsically regulated. Our results show that PHF13 can oligomerize via conserved ordered regions in its N- and C- terminus increasing its chromatin valence and avidity. Impressively, a 4-fold over expression of PHF13 was sufficient to globally compact chromatin, dependent on its ordered dimerizing regions and oligomerization potential. Unexpectedly, we discovered that PHF13 can self-associate independent of its ordered domains via intrinsically disordered regions, which conversely reduced PHF13’s chromatin affinity. Our findings support that there is an intrinsic balance between PHF13’s ordered and disordered regions that regulates PHF13 chromatin affinity, and suggest that PHF13 can phase transition between polymer-polymer and liquid-liquid phase separation states to impact chromatin structure and function.
Published in
Differential oligomerization regulates PHF13 chromatin affinity and function
Rossi F, Magalhaes AP, Buschow R et al. · Nucleic acids research 2025 · PMID 40598901 · doi:10.1093/nar/gkaf572
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE289538_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1223390 and SRA study SRP563736. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 18 more — browse all 18 samples with per-sample file links →

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