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High throughput identification of genetic regulators of microglial inflammatory processes in Alzheimer's disease.

GSE289721 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/12/31 Platform GPL24676
Summary
We sought to assess at scale how AD GWAS hits influence human microglial inflammatory responses. We conducted CRISPR inhibition screens of 119 AD GWAS hits in parallel along with 52 control genes in human iPSC-derived microglia, with reactive oxygen species produced in response to the viral mimic poly(I:C) as a readout. Top hits that either decreased or increased ROS in response to poly(I:C) when knocked down were then interrogated via perturb-seq.
Published in
High throughput identification of genetic regulators of microglial inflammatory processes in Alzheimer's disease
Cardona CL, Wei L, Kim J et al. · Journal of neuroinflammation 2025 · PMID 41340152 · doi:10.1186/s12974-025-03562-9
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Direct links to NCBI, no account and no request form: the whole study as GSE289721_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1224254 and SRA study SRP564165. Searching any of these in the dataset finder brings you back here.

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