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Pyruvate kinase M2 activation reprograms mitochondria in CD8 T cells, enhancing effector functions and efficacy of anti-PD1 therapy

GSE289773 Mus musculus (mouse) DNA methylation 26 samples Submitted 2025/05/01 Platform GPL21103
At a glance

DNA methylation · mouse · 26 samples

What it is
DNA methylationChemical marks on DNA that help switch genes on or off.
Organism
Mus musculus (mouse)
Samples
26 samples: 7 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Pyruvate kinase M2 activation reprograms mitochondria in CD8 T cells, enhancing effector functions and efficacy of anti-PD1 therapyCell metabolism · 2025
SuperSeries — this record groups several sub-series.
Summary (written by the submitters)
This SuperSeries is composed of the SubSeries listed below.
Published in
Pyruvate kinase M2 activation reprograms mitochondria in CD8 T cells, enhancing effector functions and efficacy of anti-PD1 therapy
Mortazavi Farsani SS, Soni J, Jin L et al. · Cell metabolism 2025 · PMID 40199327 · doi:10.1016/j.cmet.2025.03.003
This dataset
Open GSE289773 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE289773_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for beta-value tables or raw IDAT files among the supplementary files.

Also filed as BioProject PRJNA1224759 and SRA study SRP564359. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40199327) ↗
Study design
7 conditions, mostly with about 4 replicates each
CD8 T cells supplemented with 30 internationa… ×4 CD8 T cells activated with Ova peptide (0.5 μ… ×4 CD8 T cells activated with Ova peptide (0.5 μ… ×4 CD8 T cells activated with Ova peptide (0.5 μ… ×4 CD8 T cells activated with Ova peptide (0.5 μ… ×4 CD8 T cells activated with Ova peptide (0.5 μ… ×3 CD8 T cells activated with Ova peptide (0.5 μ… ×3

Supports a between-group comparison across 26 samples.

How we read this: 7 replicated groups read from 26 sample titles; they account for 26 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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