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Effect of FBL depletion on cell morphology

GSE289801 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/10/31 Platform GPL24676
Summary
This study reveals a previously unrecognized role of FBL in maintaining epithelial characteristics. Its depletion led to a marked reduction in Scribble, a key basolateral protein required for stabilizing E-cadherin at the membrane and sustaining Hippo signaling, thereby preventing TAZ activation. Scribble’s bivalent promoter is pre-associated with H3K4me3, H3K27me3, and EZH2. As a nucleolar protein, FBL retains a fraction of EZH2 within the nucleolus, and its loss results in the redistribution of EZH2 into the nucleoplasm, where it engages PRC2-bound promoters, including that of Scribble.Diminished Scribble levels upon FBL knockdown disrupted Hippo signaling, leading to TAZ accumulation and subsequent upregulation of EMT drivers Snail and Foxc2. Additionally, Scribble modulates Akt activity via PHLPP1, and its depletion enhanced Akt-dependent phosphorylation of EZH2, compromising its methyltransferase function. In MCF10A (p53 DN) cells, loss of FBL increased mesenchymal markers (Snail, Twist, Zeb1) while down
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Also filed as BioProject PRJNA1224826 and SRA study SRP564431. Searching any of these in the dataset finder brings you back here.

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