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ZDHHC12 palmitoylates HDAC8 and promotes the development of hepatocellular carcinoma associated with a diet high in saturated fatty acids

GSE290907 Homo sapiens (human) RNA-seq (gene expression) 30 samples Submitted 2025/12/03 Platform GPL11154
At a glance

RNA-seq (gene expression) · human · 30 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Topic
Hepatocellular carcinomaThis series is part of our curated briefing on it.
Samples
30 samples: 15 × high red meat diet vs 15 × normal dietGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
ZDHHC12 Palmitoylates HDAC8 to Promote the Progression of Hepatocellular Carcinoma Associated with a Diet High in Saturated Fatty AcidsAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
Summary (written by the submitters)
Excessive intake of saturated fatty acids (SFAs), which are prevalent in diets high in fried foods and red meat, poses a significant threat for hepatocellular carcinoma (HCC). Herein, we found via Mendelian randomization (MR) analysis that SFAs could be one of the factors leading to HCC. However, the role and associated mechanism of SFAs in HCC progression remain poorly understood.
Read the full summary ↓
Palmitic acid (PA) is the most common type of SFA. We therefore chose PA as an example of SFAs for our study. We identified zinc finger DHHC-type palmitoyltransferase 12 (ZDHHC12) as a key protein involved in the promotion of HCC progression by PA, which was accomplished by maintaining the stability of the recognized HCC-promoting gene histone deacetylase 8 (HDAC8). Mechanistically, supplementation with PA led to the upregulation of ZDHHC12 expression via activation of the transcription factor SWI/SNF-related BAF chromatin remodeling complex subunit ATPase 4 (SMARCA4). ZDHHC12 mediated the palmitoylation of HDAC8 at cysteine 244, thereby preventing the lysosomal degradation of HDAC8, which promoted the progression of HCC. In conclusion, this study revealed that ZDHHC12 was a key protein in palmitic acid-mediated HCC progression and that targeting HDAC8 could inhibit this process. These findings offer a potential therapeutic strategy for HCC patients with a high intake of saturated fatty acids, especially palmitic acid.
Published in
ZDHHC12 Palmitoylates HDAC8 to Promote the Progression of Hepatocellular Carcinoma Associated with a Diet High in Saturated Fatty Acids
Jin X, Hong Y, Zhao Y et al. · Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 · PMID 40787880 · doi:10.1002/advs.202505702
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE290907_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1230739 and SRA study SRP567452. Searching any of these in the dataset finder brings you back here.

Study design
15 × high red meat diet vs 15 × normal diet

Supports a between-group comparison across 30 samples.

How we read this: 2 replicated groups read from 30 sample titles; they account for 30 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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