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ChIP-seq profiling of estrogen receptor binding in MCF-7 cells exposed to 5α-DTT, Flutamide, Estradiol, and Bisphenol B

GSE291416 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/03/12 Platform GPL24676
Summary
Endocrine-disrupting chemicals (EDCs) can modulate estrogen receptor (ER) signaling by altering its transcriptional activity. To investigate these effects, we performed chromatin immunoprecipitation sequencing (ChIP-seq) on cells treated with 5α-Dihydrotestosterone (5α-DTT), Flutamide, Estradiol, and Bisphenol B. This dataset includes raw sequencing reads, processed peak files, and metadata, enabling the identification of ER binding sites influenced by each EDC. Gene ontology (GO) analysis of ChIP-enriched regions revealed that these compounds affect key pathways, including antibody-dependent cytotoxicity, BMP signaling in cardiac induction, and hepatocyte growth factor receptor signaling. This dataset provides insights into EDC-induced transcriptional regulation and contributes to the understanding of their role in endocrine disruption.
Published in
Unraveling ER dimerization dynamics in endocrine disruption based on a BRET-focused approach
Yum S, Lee H, Kwon YK et al. · Animal cells and systems 2025 · PMID 40304013 · doi:10.1080/19768354.2025.2481984
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Direct links to NCBI, no account and no request form: the whole study as GSE291416_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1233327 and SRA study SRP568749. Searching any of these in the dataset finder brings you back here.

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