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Therapeutic Potential of Regulatory T Cells for Stem Cell Regulation: Insights from Treg-Mediated Enhancement of Limbal Stem Cell Functions

GSE291731 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/04/16 Platform GPL24676
Summary
Regulatory T cells (Tregs) play a key role in immunomodulation and tissue regeneration. Limbal stem cells (LSCs) maintain corneal epithelial homeostasis, and limbal stem cell deficiency (LSCD) leads to visual impairment. Current LSCD treatments face donor shortages and graft rejection risks. The present study explored Tregs’ therapeutic potential for LSCD in a mouse model of graded LSCD, and further explore the direct effect of Tregs on LSCs function by in vitro coculturing human-derived cells. Subconjunctival Tregs injection effectively treated mild and moderate LSCD in mouse models. Coculturing human LSCs with human Tregs promotes LSCs migration, proliferation, and stemness maintenance. Furthermore, amphiregulin (AREG), secreted by Tregs, was crucial to their therapeutic effects, as AREG-/- Tregs resulted in diminished efficacy on LSCD mice compared to wild-type Tregs. These findings highlighted Tregs as a promising treatment for LSCD, enhancing LSC function partially via AREG production.
Published in
Therapeutic potential of regulatory T cells for stem cell regulation: Insights from Treg-mediated enhancement of limbal stem cell functions
Fang F, A T, Chen J et al. · iScience 2025 · PMID 40491963 · doi:10.1016/j.isci.2025.112515
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Also filed as BioProject PRJNA1235155 and SRA study SRP569851. Searching any of these in the dataset finder brings you back here.

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