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HMGN1 and HMGN2 are recruited to acetylated and histone variant H2A.Z-containing nucleosomes to regulate chromatin state and transcription

GSE291947 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/11/21 Platform GPL24247
Summary
The High Mobility Group Nucleosome-binding (HMGN) proteins are small abundant nuclear proteins that directly bind nucleosomes and constitute a major component of chromatin. HMGN proteins specifically localize to enhancers and actively transcribed genes across the genome, however the roles of HMGN proteins in regulating chromatin structure and transcription remain poorly understood. To investigate the localization and function of HMGN proteins on chromatin, we engineered mouse embryonic stem cells (mESCs) lacking HMGN1 and/or HMGN2 (Hmgn1-/- mESCs, Hmgn2-/- mESCs, and Hmgn1-/-Hmgn2-/- mESCs) and profiled gene expression and localization of architectural proteins. ChIP-sequencing was performed for the RAD21 subunit of cohesin and CTCF to assess the functional implications of loss of HMGN1 and HMGN2 on cohesin and CTCF localization.
Published in
HMGN1 and HMGN2 are recruited to acetylated and histone variant H2A.Z-containing nucleosomes to regulate chromatin state and transcription
Gohil R, Gao Z, Lewis RA et al. · The Journal of biological chemistry 2026 · PMID 41325801 · doi:10.1016/j.jbc.2025.110997
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Also filed as BioProject PRJNA1236228 and SRA study SRP570425. Searching any of these in the dataset finder brings you back here.

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