GEO series

Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function [CUT&Run]

GSE292056 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/03/15 Platform GPL21493

Summary

Genes encoding subunits of the BAF (mammalian SWI/SNF) ATP-dependent chromatin remodeling complex are among the those most enriched for deleterious de novo mutations in intellectual disabilities and autism spectrum disorder, but the causative molecular pathways are not understood. Synaptic activity in neurons is critical for learning and memory and proper neural development. While BAF is required for activity-dependent developmental processes, such as dendritic outgrowth, the immediate molecular consequences of neuronal activity on BAF complexes are unknown. Here we report that neuronal activity induces dramatic remodeling of the subunit composition of BAF complexes within 15 minutes, concurrent with both phosphorylation and dephosphorylation of its subunits. These biochemical effects are a convergent phenomenon downstream of multiple calcium-activated signaling pathways in neurons and fibroblasts and correspond to changes in BAF-dependent chromatin accessibility. Our studies imply that BAF decodes signals at the membrane by altering the combinatorial composition of its subunits.

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Also filed as BioProject PRJNA1236400 and SRA study SRP570571. Searching any of these in the dataset finder brings you back here.

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