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The DLX/Notch axis is necessary for spatiotemporal regulation of neural cell fate

GSE292442 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2026/05/21 Platform GPL24247
Summary
The neuronal-glial cell fate switch in the developing ventral telencephalon is a tightly regulated process. DLX2 is well established as important in promoting interneuron differentiation and migration but the mechanisms for repression of glial fate remain elusive. Here, the DLX2 regulatory network dynamic in the developing telencephalon was fully characterised using a multiomic approach at single-cell resolution. Using coupled snRNAseq and snATACseq, and single-cell spatial transcriptomics we identified spatiotemporal-context dependent effects of Notch repression by DLX2 in maintaining progenitor populations and facilitating neuronal differentiation. Furthermore, we showed that DLX2 directly represses Notch signalling genes and glial fate promoting transcription factors, thereby repressing early adoption of oligodendrocyte fate during neurogenesis. Thus, this demonstrates that the temporal cell fate switch is mediated by DLX2 via a multilayer gene regulatory network and redefines the complex neuronal-glial cell specification mechanisms in the developing telencephalon.
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Direct links to NCBI, no account and no request form: the whole study as GSE292442_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1238769 and SRA study SRP571861. Searching any of these in the dataset finder brings you back here.

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