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The molecular signature and regulatory network of human umbilical cord mesenchymal stromal cells as a niche for hematopoietic stem and progenitors

GSE292574 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/09/02 Platform GPL24676
Summary
UC-MSCs have been classified as an alternative niche for expanding HSPCs. However, inconsistent outcomes in hematopoietic recovery and the shift toward specific lineage differentiation were observed following co-cultures. Moreover, the underlying molecular mechanisms and regulatory roles of UC-MSCs during hematopoiesis have not been fully understood. The co-cultures of UC-MSCs UCB-CD34+ cells were established. Integrative transcriptomic analysis of UC-MSCs collected on day 3 of co-cultures has highlighted the molecular signature and comprehensive regulatory networks. The lncRNA-RNA binding protein interaction network and lncRNA cis- and trans-regulation networks were evident. The 3 gene modules and 10 hub genes were identified in the protein-protein interaction (PPI) network, including RPS16, CD74, RPL35, COX7C, RPL38, RPS28, RPS27, RPS10, TARDBP, and TOMM7. These have shed light on niche activity of UC-MSCs in regulation of cell differentiation, genomic stability, and hematopoietic supportive during co-culture with HSPCs.
Published in
Molecular signature and regulatory network of human umbilical cord mesenchymal stromal cells as a niche for hematopoietic stem cells and progenitors
Srimorkun P, Suanpan K, Atjanasuppat K et al. · Stem cells (Dayton, Ohio) 2025 · PMID 40891909 · doi:10.1093/stmcls/sxaf057
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Also filed as BioProject PRJNA1240241 and SRA study SRP572190. Searching any of these in the dataset finder brings you back here.

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