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CAF drives spheroid formation and pleural dissemination in NSCLC

GSE293572 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2026/04/02 Platform GPL28038
Summary
Pleural dissemination in non-small cell lung cancer (NSCLC) refers to the direct spread of cancer cells from the primary tumor site to the extrapulmonary thoracic cavity, where they colonize the pleura. This study aimed to investigate the impact of cancer-associated fibroblasts (CAFs) on the development of pleural dissemination in NSCLC. Through in vitro co-culture and in vivo pleural dissemination models, we identified that CAFs promote cancer cell migration, anchorage-independent growth, adhesion, invasion, and spheroid formation of cancer cells, leading to the development of pleural dissemination. RNA sequencing analysis revealed that CAFs upregulated the expression of Cellular Communication Network Factor 1 (CCN1), a matricellular protein, in cancer cells.
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Direct links to NCBI, no account and no request form: the whole study as GSE293572_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1245264 and SRA study SRP575933. Searching any of these in the dataset finder brings you back here.

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