← BioTransfer GEO Dataset Finder
GEO series

Enhanced phase separation of BRD4S derived from CDK12 inactivation-induced IPA drives cancer metastasis

GSE293829 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/11/13 Platform GPL24676
Summary
CDK12 gene is frequently deleted/mutated in human cancers and CDK12 loss associates with intronic polyadenylation (IPA). Here, we show that CDK12 deficiency induces IPA usage in BRD4 gene locus and expression of the BRD4 short form (BRD4S). Different from the full-length BRD4 (BRD4L) that possesses a C-terminal intrinsically disordered region (IDR), surprisingly, BRD4S has a greater capacity to form phase separation in a manner dependent on oligomerization mediated by a base-interacting structural domain (BID). Through CUT&Tag profiling, we identify BRD4S specifically binds to the promoters of and transcriptionally upregulates TGF-β signaling genes including TGFB2 and LTBP1. Our study identifies IPA usage at the BRD4 gene locus as a previously unrecognized mechanism responsible for BRD4S genesis. Our findings also reveal an essential role of BRD4S condensation formation and a unique ability of BRD4S to turn on TGF-β signaling gene expression.
Published in
CDK12 Deficiency Induces BRD4S to Promote Cancer Metastasis by Enhancing Phase Separation and Chromatin Engagement
Li H, Luo J, Liang H et al. · Cancer research 2026 · PMID 41289556 · doi:10.1158/0008-5472.CAN-25-1761
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE293829_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1246547 and SRA study SRP576479. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 4 more — browse all 4 samples with per-sample file links →

Similar datasets

Search all human ChIP / ATAC / CUT&Tag datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.