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Plate-based 10X Genomics-compatible single-cell RNA-sequencing based on Smart-seq3xpress

GSE294062 Homo sapiens Expression profiling by high throughput sequencing 5 samples Submitted 2025/10/14 Platform GPL34295
Summary
In this study, we developed a novel plate-based 10X-compatible (PB10X) scRNA-seq strategy. By leveraging the Smart-seq3xpress (SS3X) principle, PB10X supports single-cell indexed sorting and cDNA generation that is downstream compatible with any commercially available 10X Single Cell 5' library construction kits such as the 10X Single Cell 5' Gene Expression and 5' V(D)J library construction kits. We demonstrated PB10X's performance on Jurkat T lymphoblasts by generating gene expression and V(D)J sequencing libraries, benchmarking it against 10X and SS3X.
Published in
Plate-based 10X Genomics-compatible single-cell RNA-sequencing based on Smart-seq3xpress
Deserranno K, Callens E, Berrevoet D et al. · BMC genomics 2025 · PMID 41353535 · doi:10.1186/s12864-025-12286-2
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Also filed as BioProject PRJNA1144900 and SRA study SRP524475. Searching any of these in the dataset finder brings you back here.

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