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Bioengineered 3D human heart valve tissues with enhanced maturational properties for disease modelling and therapeutic applications

GSE294124 Homo sapiens Expression profiling by high throughput sequencing 5 samples Submitted 2026/08/07 Platform GPL24676
Summary
Therapeutic management of heart valve disease is currently hampered by a lack of mechanistic understanding of human valve development and pathobiology. Here, we develop a protocol to generate 3-dimensional human heart valve tissues from pluripotent stem cells with enhanced maturational properties closely mirroring the valve interstitial cell, resident myeloid cells and protein expression profile of native heart valves. Importantly, bioengineered heart valves transplanted subcutaneously in rats did not calcify, highlighting potential clinical utility as a cellular therapy. In parallel, using a high-throughput 96-well platform, we demonstrate that bioengineered human heart valves can be used for modelling valve disease. Treatment with inflammatory cytokines augmented tissue passive tension and upregulated molecular markers associated with valve calcification and fibrosis. Thus, bioengineered human heart valve tissues provide a platform to understand human heart valve development, disease pathogenesis and offer a potential regenerative therapy for heart valve disease.
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Direct links to NCBI, no account and no request form: the whole study as GSE294124_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 5 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1248416 and SRA study SRP577418. Searching any of these in the dataset finder brings you back here.

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